1372 Modulation of YBX1 phosphorylation determines epidermal stem cell function

The RNA-binding protein YBX1 (Y-box binding protein-1) is an epidermal stem cell function regulator. Loss of expression in keratinocytes results a decrease the proliferative potential progenitors vitro and alterations all lineages vivo. binds 3’UTR multiple targets cells, inhibits translation potent senescence-associated cytokines guarding cells against occurrence senescence, mediates cells' self-renewal proliferation. Previous studies have shown that phosphorylation can diminish its RNA affinity. Here we show phosphorylated isolated from donors different ages elevated. Small molecule inhibitors PI3 kinase (PI3K), such as PI-103 GDC-0941 inhibit levels consequently reduce cellular senescence primary adult donors. Furthermore, PI3K increase speed wound closure healing model regeneration utilizing 3D skin equivalents. Our findings uncovered new level posttranscriptional control aging by YBX1, which required to maintain regenerative potential.